In a telephonic meeting, can I ask "Who is this?"

In our tele conference, with our Manager, we sometimes get confused who is on the other side. In such a scenario, can I ask “Who is this?”.
Please observe that we are communicating with our Manager and some Clients (who are very import).
Thanks,
Suresh

Hi,

It would perhaps be more polite to ask: Who is this speaking, please?

Alan

By the way the clients are important.